Scientific Validations Use of Ozone
The FDA (Food & Drugs Administration), the USDA (US Department of Agriculture) and the EPA (Environmental Protection Agency) have approved Ozone as an antimicrobial agent "GRAS", the USDA and the National Organic Program have also approved as an active ingredient for the sanitization of surfaces (plastic and stainless steel) in direct contact with food without the need for rinsing and with no chemical residue. OZONE HAS BEEN RECOGNIZED BY THE MINISTRY OF HEALTH (PROTOCOL N. 24482 OF 31 JULY 1996) NATURAL PROTECTION FOR THE STERILIZATION OF ENVIRONMENTS CONTAMINATED BY BACTERIA, VIRUSES, SPORES ETC. AND INFESTED WITH MITES, INSECTS, ETC. From the data obtained from a research carried out at the University of Trieste - Department of Life Sciences (D4 Rizoma project 2007-2008), it is possible to notice a reduction of the microbial load of over 90% with concentrations not lower than 2 ppm for at least 6 hours of treatment. At higher concentrations the same result was obtained by decreasing the treatment time. According to studies carried out by the University of Pavia, Dept. of Pharmacological Physiological Sciences in 2004, in a 115 cubic m room treated with ozonation for 20 minutes the bacterial load of the air was reduced by 63% and that of yeasts and molds by 46.5%, while the bacterial load of the surfaces has been reduced by 90% and that of yeasts and molds by 99%. PROTOCOLS RELEASED IN ITALY: • University of Naples “Federico II” in vitro tests of the inactivating power of nascent oxygen towards pathogenic enterobacteria and absence of genetic mutations • University of Udine - Department of food sciences prot. 219/94 decontamination tests on flat surfaces of equipment used for processing salmonella meats - listerie • University of Parma - Institute of microbiology tests to verify the sterilization capacity on bacterial colonies and. Coli s.aureus - ps.augugosa - str duranS • Ministry of Health Higher Institute of Health - Department of Veterinary Nutrition and Nutrition, protocols filed certifications, protocol 24482 31/07/96